1gtt] DACHNOWSKI—CRANBERRY ISLAND 17 
in station II. The gas is largely hydrogen gas and CO,; the latter, 
with the exception of stations V and VIII, is present usually in 
small quantities and was distinguished from other gases by its 
absorption in sodium hydroxide. Fermentation action is shown 
better on dextrose and lactose. There is little growth and gas 
formation in station I; no acid is produced in stations VII and 
IX; and very little hydrogen gas is formed in station VIII. In 
all cases the growth of the organisms produces a marked and 
varied pigmentation in the solutions. 
In plain milk, rapid coagulation precedes further bacterial 
action in all cases except station IX, in which coagulation occurs 
very slowly. Milk is slowly peptonized anaerobically in stations 
IV, V, and VI; surface digestion takes place in stations III, VIII, 
IX, and X; it is rapid in stations I, III, and VI; and gas is pro- 
duced in moderate quantities in all stations except station VIII. 
Litmus milk is coagulated in all stations; the medium gradually 
decolorizes and the cultures become acid in various degrees; the 
color does not return upon steaming the test tubes. With a 
majority, gas is produced in various amounts during digestion, 
except in station [X, in which the bacterial reaction is faint though 
strongly odorous. 
On bouillon bacterial growth is slow; it is never very turbid 
or heavily clouded, and only in one case, station IX, gives a 
whitish precipitate. 
The power of indol production is greatest with the organisms 
in stations III, V, and IX; the action is relatively small in stations 
II, IV, VI, and VII; and present to a feeble extent only in stations 
I, VIII, and X when tested with 0.02 per cent solution of potas- 
sium nitrite and sulphuric acid. 
The ability to form nitrites from nitrates in nitrate broth is 
common to the organisms in all stations. The amount of nitrites 
formed is high in stations IV, VI, IX, and X, and very small in 
stations I, 7, VII, and VIII. The power to reduce nitrates to 
nitrites is not present in the same degree as noted above for the 
reduction action in starch media. It is certain that the micro- 
organisms are capable of reducing nitrates, but to some extent 
metabolic products, apparently, modify the action. The test 
