Tort] PRITCHARD—DISSEMINATION OF PUCCINIA 177 
In experiments 5~13 inclusive, the inoculated barberry branches 
were heavily infected the 14th of May. Only spermagonia were 
present on this date, but they were frequently on both surfaces 
of the leaves. There were a few spermagonia on other parts of 
the bushes, but no such dense blotches as appeared where the rusty 
straw was applied. 
Further inoculations were made upon the barberry by scraping 
the teleutospores from dead straw, wintered the same as that 
described for Table I, and placing it on young buds or leaves which 
were carefully marked off by India ink and — These results 
are recorded in the following table. 
TABLE II 
INOCULATIONS MADE UPON Berberis vulgaris WITH TELEUTOSPORES FROM Puccinia 
graminis Pers. 
Nambarof | Date of | source of matrt | Number ts - pag 
1G eo ee May 8, 1906 Oats to buds ., Io negative 
Or an May 9, 1906 Ely. trit. 10 buds 2 positive 
Drier mine May 21, 1906 | Ber. vul. 10 buds Io negative 
Mc eee eee May 22, 1906 | Wheat 8 leaves 8 positive 
S25 a ae June 26, 1906 | Ely. trit. I 
SS June 26, 1906 | Hord. jub. 1 leaf negative 
As shown by the table, these results were mainly negative, 
perhaps on account of poor germination, since the buds selected 
were fully as young as those used earlier. 
In order to obtain pure cultures of aecidiospores of known 
origin for further experiments relative to their infecting power, 
inoculations were made upon barberry bushes which had been 
standing in the greenhouse in large tubs for nearly a year and had 
borne no rust. The infections were made by tying small pieces 
of old rusty straw on the bushes in places offering the least oppor- 
tunity for contamination and moistening the straw frequently with 
distilled water. A summary of the results is shown in Table HI. 
Aecidia appeared only on parts of the barberry inoculated, 
and, with the exception of the rusty oat straw from which all my 
inoculations so far have failed, infections were obtained from 
each kind of material. This with the data of the two preceding 
