252 BOTANICAL GAZETTE [OCTOBER 
of prickly pear, with the radicles thrust into cavities in the soft 
tissue. The bean was held in place by a setting of plaster of 
Paris, and a moist strip of cloth was brought from a vessel of water 
to furnish moisture until the plants should become established in 
their new relations. 
May 13.—A large number of new insertions of the “‘rusty”’ 
bean in joints of Opuntia and bodies of Carnegiea and Echinocactus 
were made on this day. Some of the original preparations were 
showing notable growth. 
May 14.—Some of the plants first arranged had thrust the tips 
of the plumules beyond the cotyledons, but had not yet straight- 
ened the hypocotyl. One bean with wilted plumule was taken 
out of the Opuntia. The main root had grown but little. A 
secondary branch from near its base had come out and extended . 
down into the cavity, alongside the main root, showing as great 
a length. 
May 22.—Twelve seedlings on Opuntia had survived, of which 
one had developed the plumule to an extent that the leaves were 
unfolding from between the cotyledons. Six seedlings on Echino- 
cactus had survived, and were showing some slight development. 
Three seedlings out of g insertions on Carnegica had survived and 
had made-.a slight development. The cotyledonary curvature 
was still markedly present. 
May 31.—Temperatures of 111° and 112° F. out of doors, and 
all parasites were flagging, apparently dying. All soon perished. 
About a dozen germinated beans of the form known as ‘small 
Papago white” were inserted in joint of ‘‘Mission Pear” (Opuntia 
sp.), near the laboratory at Carmel, California, on June 23, without 
any protection except a cloth shade. These, with the exception 
of two, dried out, although small secondary roots were formed by 
June 27. All were replaced and a small vial was arranged with a 
strip of cloth to give shade and moisture. About 8 similar preP- 
arations were arranged on Oenothera biennis and O.H 206. On 
July 1, only one of this lot had become dry, the fogs of the pre- 
ceding days having been an obvious advantage. 
July 14.—Seven Papago white beans on Mission Pear thriving. 
of which three had well developed leaves of the first simple pair, 
