280 BOTANICAL GAZETTE [ocroBER 
ever, the daughter nuclei reorganize so close together that after a 
slight growth they are pressed against each other and resemble 
fusing nuclei (fig. 13). The spindle fibers are frequently present 
at this stage, and can be seen connecting the two masses of chro- 
matin, which are still visible in the daughter nuclei. Frequently 
the masses of chromatin lie against the nuclear membrane, and the 
disappearing fibers are entirely outside the nucleus, but at other 
times the fibers appear to cross the nuclear cavity, as in fig. 13. 
Frequently the chromatin appears, at first sight, to be in the center 
of the nucleus, when in reality it is lying against the membrane. 
This is due, of course, to the fact that the chromatin is at the upper 
or lower surface of the nucleus as it is viewed from above. 
Division seems to take place rapidly throughout the growth of 
the ascogonium and the development of the ascogenous hyphae. 
The nuclei do not divide simultaneously, as all stages, including 
resting nuclei, can be found in a single ascogonium. There appear, 
however, to be periods in which division takes place, followed by 
others in which all of the nuclei are in the resting condition, for a 
large number of divisions are frequently found in a single ascogo- 
nium, while others show only resting nuclei. The same type of 
division that has just been described and the same number of 
chromosomes persist throughout the development of the ascogonium 
and ascogenous hyphae. The nuclei decrease somewhat in size 
during the growth of the ascogonium, and in the early stages of the 
development of the ascogenous hyphae, but as the ascogenous 
hyphae develop further, the nuclei increase in size until they come 
to be slightly larger than in the young ascogonium. 
No fusion of nuclei has been observed in the ascogonium or in 
the ascogenous hyphae except in the tips where two nuclei fuse to 
form the primary nucleus of the ascus. A number of cases were 
seen in which two nuclei were pressed against each other, but in 
all of these the nuclear membrane was intact between the nuclei, 
and the appearance seemed to be due to the fact that the nuclei, 
_ after division, had reorganized close together, in the manner pre- 
viously described. It may be said that a fusion of the nuclei would 
be hard to find, but they have been looked for very carefully ina 
large number of well fixed and stained preparations. The slight 
