290 BOTANICAL GAZETTE {OCTOBER 
When the five chromosomes have become connected with the 
centrosome, other deeply staining bodies are frequently present 
on the linin fibers. These are usually small and are probably 
similar to the granules previously described. Sometimes, however, 
they are as large as or larger than the chromosomes, and may bear 
such a striking likeness to them that there may appear to be as 
many as six or seven chromosomes (fig. 36). When the spindle 
is completely formed, these bodies may still be present on fibers 
connected with the spindle or nucleolus. Only very small ones, 
however, have been seen on the spindle, so that when the spindle 
is formed these bodies, which usually stain lighter than the chromo- 
somes; can be readily distinguished from them. 
After the linin fibers have become connected with the cen- 
trosome, they increase in number. The centrosome then divides, 
and the two daughter centrosomes take positions at the opposite 
ends of the spindle (figs. 37 and 38). When the spindle is first 
formed, it may be at any angle to the longitudinal axis of the 
ascus, but as division proceeds, it takes a position which is approxi- 
mately parallel to it. While this is taking place, a set of fibers 
makes its appearance outside the nucleus. These fibers radiate 
from the centrosome into the cytoplasm for a considerable distance. 
At metaphase five chromosomes are present on the spindle (fig. 
38). Usually all of these appear to be somewhat elongated and 
have their longitudinal axis parallel to that of the spindle. Each 
of the five chromosomes divides transversely, but the divisions do 
not all take place at the same time, so that as division proceeds, 
anywhere from six to ten chromosomes may be counted on the 
spindle. Remembering that when the spireme segmented it gave 
rise to five elongated chromosomes which were probably bivalent, 
it would seem that this division probably separates chromosomes 
which were placed end to end on the spireme and can have nothing 
to do with the longitudinal split seen in the prophase. There 
appears to be nothing to indicate that the chromosomes which 
went into the fusion nucleus have persisted unchanged through the 
resting nucleus and the prophases of this division, and are the same 
as the chromosomes which are separated at metaphase. On the 
contrary, there would seem to have been every chance for an 
