272 BOTANICAL GAZETTE [APRIL 
to give it the same compactness as the soil of the field. Thirty 
kilograms of soil were put into each can. 
The seedlings which had 3 or 4small leaves were planted in th 
tanks on November 27, 1908. At that time each plant was given 
500 cc. of water at the surface, and 1000 cc. were added to each 
tank through the inlet tube. The tanks were left under cover of 
the shed until the following day (November 28), when all the plants 
had completely recovered from the effects of transplanting. The 
tanks were then given another liter of water through the inlet tube 
and enough more was added to those that required it to bring them 
all to the same weight. 
One set of six tanks with plants, and three without plants that 
had been treated in every way like those containing plants, was 
placed in two rows among the plants of the regular crop under the 
cheese-cloth which covered an area of one hectare (2.471 acres). 
‘The other similar set was placed in like manner in an adjoining 
field of one hectare also planted with tobacco. The sets of experi- 
mental plants were thus subjected to the same conditions in their 
respective environments as the plants of the regular crop. 
The general development of the experimental plants was in 
every way normal, and did not differ from that of those among 
which they stood. The axillary buds were removed as soon as they 
appeared, so that the plants grew to a single stem without branches. 
The terminal buds were not removed, however, as is customary 10 
commercial practice. 
The shade plants attained a nearly uniform height of 2.1 
meters, while the height of the sun plants, which were a little less 
uniform, averaged about 1.75 meters. The leaves of the shade 
plants were much larger and thinner than those of the sun plants 
and the internodes of the stems were longer. 
During the course of the experiment the original seedling leaves, 
which had increased much in size, withered. These were cut © 
and dried and later were ground up with the rest of the plants from 
. which they had been taken. 
In spite of the tamping of the soil in the tanks, it was found that 
the soil water receded from the upper layers of soil, which became 
very dry. Whenever this condition led to incipient wilting, 
