82 BOTANICAL GAZETTE [aucust 
R 
chiefly anatomical. Miss Kitpant’s work was done in this laboratory, | 
and I have had the opportunity of examining her preparations. The 
receipt of additional material has made it possible to continue the 
study and to fill in some of the missing stages. This work, a previo 
study of Dacrydium, and the opportunity of examining some mater 
of Podocar pus have given occasion for the present paper. I shall 
consider Phyllocladus, and later take up the larger question of the 
relationships of the Podocarpineae. . 
I. Phyllocladus E 
The material sent by Dr. L. Cockayne of Christchurch, New — 
Zealand, consisted of staminate cones collected at intervals of a few 
days from October 16 to November 13, and ovulate cones of Novem 
ber 8, 13, 25, December 3, 18, 31, January 8, 28. The jron-alum 
hematoxylin and orange combination was found very satisfactor, 
in staining, as it brought out cytological details remarkably well for 
tissues killed in formalin and alcohol. 
THE MALE GAMETOPHYTE : 
Miss Kiipaut reports (1) the formation of two prothallial cells 
the first of which is usually evanescent; (2) the presence of four, _ 
occasionally five, free nuclei in the mature pollen grain; and (3 
the division of the body cell into two equal male cells. 
as cavities in the exine. The intine is usually rather thin. Ve e 
small starch grains occur, but are never conspicuous. Figs. 1-5 show 
the cutting off of the prothallial cells. The first usually degenera 
so quickly that it is hard to find in the older grains. In fig. 4 t® 
seen partly covered by exine; this is an oblique section escaping the 
wings and making the spore coats appear uncommonly thick. - 
clinally, as in the case of Ginkgo, Podocarpus, and Dacrydium, a0@ 
presumably in all the Podocarpineae. The four-celled stage, 
shown in fig. 7, but for the wings might very easily be mistaken f 
the shedding stage in Ginkgo. In the latter, it will be remem 
