186 BOTANICAL GAZETTE [SEPTEMBER 
and the other without calcium carbonate, they were stored at 
o° C. for 34 days. At the end of this time, 200 cc. of water were 
TABLE I 
Loss OF CATALASE DUE TO ACIDS IN THE EXTRACT 
cc. O, EVOLVED IN I-MIN. PERIODS | Torat cc. O» 
Potato IN 5 MIN. 
1st 2d 3d 4th 5th | S 
Ground without CaCO;....| 3.2 3.0 2.3 7 r.3 eo ee 
Same extract after standing 
bus i | ae EN Omg uy ae Fi7 1.8 1.6 7.4 1.0 7-4 
Ground with CaCO,....... 6.4 5.0 4.6 3.6 2.8 23-3 
Same extract after standing ; 
MBE s So eas 5 5.8 Aas a8 2.6 2355 
added to each flask containing the pulp, and 5 cc. of this dilution 
and 5 cc. of hydrogen peroxid were used for the determination in 
each case. 
TABLE II 
EFFECT OF STORAGE FOR 34 DAYS AT 0° C. WITH AND WITHOUT CALCIUM CARBONATE 
cc. O, EVOLVED IN 30-SEC. PERIODS Torat cc. 0: 
Potato ; IN 3 : 
; 1st 2d 3d 4th 5th 6th 
a eee 
Ground without 
A ee 3 4 0.4 0.4 °.4 0.4 2.3 
Ground with CaCO, 12.4 | 10 9 8.9 6.9 C4 4.0 48.4 
FILTERING 
Approximately 50 per cent of the total catalase will pass through 
an ordinary filter paper, but none through a Chamberland-Pasteur 
filter. The first fact seems to indicate the existence of an insol- 
TABLE III 
EFFECT OF FILTERING THROUGH ORDINARY. FILTER PAPER 
cc. 0, EVOLVED IN 30-SEC. PERIODS Torat cc. 02 
EXTRACT OF POTATO TUBER - IN 3 MIN. 
2d 3d’ | ath | 5th | 6th 
Ist © 
Portion of same 
tered 
ee ore a ay ee Heres] 4.0 1 4.5 3:8 sn 
a arena maT Tear ge on oe NR a 
Filtered through filter paper ....| 2.8 COP lly on ea ge ae ie ee 10.9 
Since wie xa 
wee aia a 
uble (-catalase) and a soluble (8-catalase) form. Kout finds the - 
same true of yeast catalase, and Loew believes it a general chat” 
