E 
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STAs 
{gi ee Apa 
gto] BROW N—ASCOCARP OF LEOTIA 447 
Lachnea scutellata the nuclei of the storage cells probably do not 
usually fuse. 
The details in the formation of the asci were studied chiefly 
in Leotia chlorocephala, and will be described in connection with 
that species. The appearance of the hymenium suggests, however, 
that the process is similar in the two cases. 
Leotia chlorocephala 
The ascogenous hyphae in Leotia chlorocephala, as in L. lubrica, 
have their origin in the stipe. The hymenium is also formed in 
the same way that has been described for L. lubrica. 
In 1894 DANGEARD described the asci of Peziza vesiculosa as . 
originating from a binucleate penultimate cell of an ascogenous 
ypha. Since that time the same phenomenon has been described 
by various workers in a number of forms. A large number of 
these are mentioned by OVERTON (23). The usual case seems to be 
that there are two nuclei in the tip of an ascogenous hypha. These " 
divide simultaneously and walls come in between the sister nuclei 
of the two pairs, thus forming a binucleate penultimate cell and 
@ uninucleate ultimate and antepenultimate cell. The ascus is 
then formed from the binucleate penultimate cell. Marre (20) 
and GUILLIERMOND (14) have described the asci of Galactinia 
succosa as originating from a binucleate ultimate cell. In Humaria 
granulata (BLACKMAN and FRASER 4) the asci usually arise from a 
binucleate penultimate cell, but in two cases the asci had a terminal 
position. 
In speaking of L. chlorocephala and a number of other asco- 
mycetes, Fauii (11) says: ‘“‘It is quite probable that the only 
cell; fig. 26, hook in which two nuclei have fused to form nucleus of ascus, and tip 
has fused with stalk of hook; fig. 27, ultimate cell has fused with antepenultimate; 
nucleus of latter has migrated into former, which is growing out to give rise to 
or another hook; fig. 28, two nuclei of eaves cell have fused to form nucleus 
of ascus; aitiionte cell has fused with antepenultimate, and nucleus of latter has 
migrated into former, which has grown oars form another hook; fig. 29, binucleate 
Penultimate cell has given rise to hook; ultimate cell has fused with penultimate, 
and the two nuclei have fused; ultimate cell has not developed further; fig. = bi- 
nucleate penultimate cell has formed ascus, which fusion produc ct of ultimate and 
antepenultimate has given rise to second ascus; fig. 31, illustrating oe 
Plication of number of asci by method shown in figs. 26-30; figs. 9-20, 1400; figs. 
_ 21-30, X 2100. 
