SCOTT, STUDY OF CHANGES IN MVSTELUS CANI8 



13 



sample of blood. The results are as useful for purposes of comparison as 

 if the blood had first been defibrinated and then centrifuged. Time was 

 saved by omitting these procedures and I believe that the results are as 

 satisfactory. The actual pressure in atmospheres can be easily found by 



22 4 



multiplying A by -^ ( = 12.108). 



1.8o 

 EFFECT OF FRESH WATER 



After the first sample of blood was taken, the animal was placed in a 

 tank of sea-water, into which fresh water was then run, so that in a few 

 minutes the water in the tank was fresh. The results of this experiment 

 were obtained from a series of ten fishes, six males and four females, 

 ranging from 61 to 82 centimeters in length, and are shown in Table 

 VII. 



Table VII. — Changes in the freezing point of the Mood of Mustelus canis after 

 immersion in fresh water until nearly dead 



Sex 



Length in 

 cm. 



Weight in 

 grams 



Immersion 

 time in 

 minutes 



A of blood 



Change in A 

 of blood 



9 



79 



1588 





 15 

 30 

 45 

 60 

 73 



1.93 ° 



1.905 



1.805 



1.705 



1.630 



1.600 



0.000° 



0.025 



0.125 



0.225 



0.300 



0.330 



d" 



64 



822 





 25 



60 

 80 



1.81 

 1.805 

 1.530 

 1.380 



0.000 

 0.005 

 0.280 

 0.430 



? 



69 



1021 





 40 

 65 

 90 



1.88 

 1.73 

 1.64 

 1.435 



0.000 

 0.150 

 0.24 

 0.445 



9 



61 



652 





 20 

 45 

 60 



1.87 

 1.81 

 1.64 

 1.50 



0.000 

 0.06 

 0.23 

 0.37 



d 



82 



2211 





 15 

 30 

 45 

 60 



1.880 



1.855 



1.79 



1.71 



1.61 



0.000 



0.025 



0.09 



0.17 



0.27 



& 



74 



1134 





 20 

 40 

 60 



80 



1.880 



1.84 



1.74 



1.64 



1.38 



0.000 



0.04 



0.14 



0.24 



0.50 



