14 



ANNALS NEW YORK ACADEMY OF SCIENCES 

 Table VII. — Changes in the freezing point — (Continued.) 



Sex 



Length in 

 cm. 



Weight in 

 granns 



Immersion 

 time in 

 minutes 



A of blood 



Change in A 

 of blood 



? 



80 



1687 





 35 

 55 



70 



85 



1.890 



1.76 



1.605 



1.53 



1.39 



0.000 



0.13 



0.285 



0.36 



0.50 



C^ 



77 



1502 





 20 

 40 

 55 



1.900 

 1.84 

 1.74 

 1.59 



0.000 

 0.06 

 0.16 

 0.31 



d 



79 



1460 





 15 

 30 

 45 

 65 

 75 



1.850 



1.81 



1.76 



1.635 



1.50 



1.40 



0.000 



0.04 



0.09 



0.215 



0.35 



0.45 



? 



76 



1304 





 15 

 40 

 55 

 70 

 80 



1.920 



1.87 



1.74 



1.63 



1.47 



1.44 



0.000 



0.05 



0.18 



0.29 



0.45 



0.48 



In averaging the results, we may divide the time into five periods of 

 twenty minutes each, the first twenty minutes of immersion constituting 

 the first period and so on. The average cliange during each period of 

 immersion is as follows : 



1st twenty minutes = +0.050° -^ 



2nd twentyminutes= 4"0-133 

 3rd twenty minutes = -1-0.265 

 4th twenty minutes = -f 0-400 

 5th twenty minutes = -j-0.470 



The average of the ten maximum determinations is -[-0.408°. 



As was found in the series of experiments described on page 9 there 

 are indications here also of individual variations in the reaction of the 

 fishes to the changed environment. Figure 1 is a curve which represents 

 the course of the change in the depression of the freezing point and there- 

 fore a fall in the osmotic pressure of the blood from the beginning to the 

 end of the experiment. This curve is derived from the values computed 

 for each of the twenty minute periods. Certain features of this curve 

 may be here pointed out. There is a slow change at the beginning of the 

 experiment. This continues during the first two of the five periods of 

 immersion. There is then a change in the slope of the curve, indicating 



