16 



ANNALS NEW YORK ACADEMY OF SCIENCES 



tained. The procedure was in the main as before. The tank in which 

 the specimens were placed after the operation contained about twenty- 

 four liters of sea-water. To increase the amount of salts in solution in 

 this sea- water, about 500 grams of sea-salt were dissolved in a jar con- 

 taining eight liters of sea-water. This was placed above the tank. After 

 the normal sample of blood was obtained, the specimen in each case was 

 placed in the tank of sea-water and the concentrated solution from the 

 jar was at once run into the tank at one end, the overflow running out at 

 the other end. At the same time, the various samples of blood were ob- 

 tained for the determinations of the freezing point, the specific gravity 

 of the water in the tank was taken. On the whole, the specific gravity of 

 the solution was 1.0344-. Its A was about 2.60°. The A of the sea- 

 water was about 1.82° and its specific gravity, 1.025. An analysis of the 

 chlorides in both sea-water and in water of the concentration attained at 

 the end of each of these experiments showed that the latter contained 

 about 33 per cent more salts than sea-water. The water in the tank 

 reached this concentration in about fifteen minutes after each experiment 

 began. The results are given in Table VIII. Data with regard to eleven 

 specimens are shown, seven females and three males, ranging in length 

 from 67 cm. to 84 cm. The sex of one animal was not recorded. 



Table VIII. — Changes hi the freezing point of the hlood of Miistelus canis after 

 immersion in a concentrated solution of sea-icater nntil near death 



Sex 



Length in 

 em. 



Weight in 

 grams 



Immersion 

 time in 

 minutes 



/\ of blood 



Change in A 

 of blood 



9 



80 



1531 





 17 

 30 



48 

 65 



77 



"^1.84° 

 1.90 

 1.96 

 1.99 

 2.06 

 2.08 



0.000° 



0.06 



0.12 



0.15 



0.22 



0.24 



? 



80 



1361 





 20 

 35 

 50 

 65 

 80 



1.84 

 1.93 

 2.01 

 2.05 

 2.11 

 2.15 



0.000 



0.09 



0.17 



0.21 



0.27 



0.31 



d" 



75 



1247 





 25 

 40 

 55 

 75 



1.88 

 1.94 

 2.00 

 2.07 

 2.10 



0.000 



0.06 



0.12 



0.19 



0.22 



? 



67 



950 





 15 

 30 

 50 

 65 



1.80 

 1.87 

 1.94 

 2.00 

 2.08 



0.000 



0.07 



0.14 



0.20 



0.28 



