42 



ANNALS NEW YORK ACADEMY OF SCIENCES 



Experiment 9. Mackerel, Scomljer scombrus. 10 inches long. 



No laking in 2.0 per cent to 0.8 per cent NaCl. Distinct in 0.6 per cent 

 NaCl. Decided in 0.4 per cent NaCl. 

 Experiment 10. Butterfish. 



No laking in 2.0 per cent NaCl to 0.8 per cent NaCl. Laked in 0.6 per cent 

 NaCl. 



According to Eodier and Quinton the blood of marine teleosts contains 

 about 0.6 per cent salts, while that of elasmobranchs has about 1.7 per 

 cent. I have found that the blood serum of Mustelus contains .86 per 

 cent CI, while that of the blood of the flounder, Pleuronectes, a marine 

 teleost, has .53 per cent CI. The equivalent in NaCl for the dog-fish is 

 1.42 p^r cent, while for the flounder it is 0.87 per cent; and yet we have 



Solutions 



of 



NaCl. 



2.0^ 

 1.8" 





a s: 

 ^1 



1^ 

 11 





1^ 



1 



06 ^ 



s 

 1 



1 



1 























1.6" 























1.4" 























1.2" 























1.0" 

 0.8" 

 0.6" 

 0.4" 



a2" 



«<• •• 



• •• • • 



1 



• « ••• 



• • • «« 



• •• • % 



• •4 4* 



■ 



'i'trnfi 



*»»»* 



1 





1 







1 



'««. « 



*.»»*» 



^^^ 



1 



ll 



k«>«^ 





■1 



0.0" 



H 



1 



■ 



■ 



1 



■ 



■■ 



■ 





^TT 



^ 



* 



5 



^ 



^ 



^rn 



Tl 



Fig. 9. — Showing the hemolytic effect of different NaCl solutions on the erythrocytes of 

 four species of elasmobranchs and six species of teleosts. Nos. 1-^ ::= elasmobranchs ; 

 5-10=^ teleosts. Blank spaces, no laking; dotted spaces, faint laking; dark spa^ces, 

 decided laking. 



found that in the spiny dog-fish the first decided laking occurred in the 

 0.6 per cent solution of NaCl; in the other three elasmobranchs, in the 

 0.8 per cent NaCl solutions. In three marine teleosts studied the first 

 decided laking occurred in the 0.6 per cent solutions, while in the other 

 three species decided laking first occurred in the 0.4 per cent NaCl solu- 

 tion. Fig. 9 shows the hgemolytic effect of different NaCl solutions on the 



