31 



(3) Leucocytozoon of Ptiiofis fusca. — We have previously 

 recorded ''free and intra-corpuscular trypanosomes" from 

 <^ueensland birds of this species. In a bird shot near Grafton, 

 New South Wales, in April, 1912, both trypanosomes and 

 Leiicocytozoa were detected. This was the first New South 

 Wales bird in which either of these parasites had been found. 



(4) Leucocytozoon of Acanthogenys rufigiilaris (Spiny- 

 cheeked Honey-eater). — In a bird shot at Merrool, near Yanco, 

 in October, 1912, these Leucocj/fozoa were detected. In young 

 forms the host-nucleus was bayed by the parasite, in older 

 forms it lay as a cap or a band on the parasite. The coloura- 

 tion of the host-nucleus by Giemsa's stain in infected cells was 

 more purplish than in the case of non-parasitized cells, the tint 

 being the same as is obtained when a red corpuscle has been 

 partly injured in making a film. In some of the largest forms 

 the protoplasm of the parasites contained a few dark granules. 

 The infection of this bird is of considerable interest. The 

 locality is in the south of New South Wales, not very far from 

 the Victorian border, the only other bird in this State that 

 we have so far found infested with the Leucocytozoon having 

 come from the opposite, north-east, corner — viz., Grafton. The 

 bird is also more of an interior, dry-loving species, whereas 

 Grafton is sub-tropical. 



IV. — Trypanosomes. 



One new infected Australian species of bird has been met 

 with since our last report. The species of trypanosome is 

 probably the same as that found in other Australian species 

 and called by us Trypanosoma anellohiae : — 



(1) Trypanosome oi Myzomela sanguineolenta (Sanguineous 

 Honey-eater). — We have previously recorded the presence of 

 Leucocytozoa ("Intra-corpuscular Trypanosomes") in this 

 species from Eidsvold, Queensland. In October, 1911, Dr. 

 Bancroft kindly forwarded another specimen from the same 

 locality, in which a few trypanosomes, as well as Leucocytozoa.^ 

 Haltendia, and Microflariae were detected. The posterior 

 end of the only uninjured trypanosome seen was finely attenu- 

 ated with the micro-nucleus 12 /ut, from the extremity. The 

 micro-nucleus, 2*5 fx long, was 11 /a anteriorly, the body at 

 this level being 5 jm wide. From the macro-nucleus the para- 

 site gradually tapered anteriorly to end at 26 /x. There was a 

 broad, undulating membrane. This is another instance o^ the 

 association of Ljcucocytozoa and trypanosomes. 



(2) Trypanosome of Ptilotis fusca. — We have previously" 

 recorded Leucocytozoon and trypanosomes from Queensland 

 specimens of this bird. The bird shot at Grafton in April, 

 1912, referred to under Leucocytozoon, also contained trypano- 

 somes. 



