I 
36 BOTANICAL GAZETTE [JANUARY 
The storage substances of the resting seed are mentioned here, 
nucellus, endosperm wall, and 
hypocotyl cap, but none was 
found in the endosperm or em- 
bryo. The embryo and endo- 
sperm are stored with an abund- 
ance of protein and fat, and also 
a trace of glucose. No starch 
was found in either endosperm 
or embryo. Histidine, tyrosine, 
and arginine are found in both 
endosperm and embryo. There 
is also a trace of leucine and 
probably cystine. 
Catalase activity of the em- 
bryo and endosperm is low, 
while that of the coat is negli- 
gible. The seed shows peroxi- 
dase activity, with a mere trace 
of oxidase activity. 
The resting seed embryo has 
a P, value of about 8, while the g: 
endosperm has a P, value of spore membrane; &, endosperm wall; 
about 5. Thus the embryo is ” Sh ee. 
basic, while the endosperm is acid, a condition opposite to that 
usually found in seeds which are ready for germination. 
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Treatment of material 
After collection the larger part of the fruit or berry was removed 
from the seed by running the berries through a coffee mill so wide 
open as not to injure the seed. Next the seeds were sifted and 
the milling and sifting repeated. The seed material was then 
\ rubbed between two sieves in the presence of an abundance of 
ater. In this way all the berry and excess tissues which prevent 
