1921] PACK—JUNIPERUS 47 
using phenolphthalein as the indicator. To show that this increased 
acid content is real the calculated dry weight of the seed material 
used is given. 
The fat of the dry seeds is stored as very large globules, con- 
trary to the statements of CzapEK, which divide and become 
continually smaller as after-ripening goes on. Just preceding 
germination these fat globules, in the active growing cells, become 
reduced to microscopic size, although CzAPEK states that the micro- 
scopically divided fat of dry seeds collects into globules with early 
TABLE XIII 
INCREASE OF TITRATABLE ACID IN ENDOSPERM AND EMBRYO 
Increased acid per 
Condition No. | Dry weight in gm.| N/50 NaOH in cc.| unit — of 
water 
COUP Oe. Sas ou 80 0.165 Ce Taye 
DO a a es 160 0.348 BG ae bee cs wale ae 
DR eee a 80 o.160 Oe Oey eats 
FIND Sch wes tyke eee a 80 0.164 0.55 0.0254 
After 15 days at 5°C....... 80 0.163 0.57 2.0989 
After 30 days at 5°C....... 0.172 ge EAE ea Oise gle sop 
After 30.days at 5°C....... 80 0.172 0.60 0.0866 
After 60 days at 5°C....... 0.169 696. Fae. 
After 60 days at 5°C....... 80 0,167 0.70 0.0685 
After 95 days at 5°C....... 80 0.157 OBhe Tee eee 
After 95 days at 5°C.......] 80 0.163 0.90 0.0564 
oniie a OE ET 80 0.189 1.50 0.1426 
Hypocotyl 2mm........... 80 0.195 2.60 0.1615 
a eo 80 0.210 8.00 een ee aes 
OO se 80 0. 204 8.00 | 0. 1865 
growth (7). This dispersion of the fatty material brings clearly 
into play surface tension, adsorption power, and many other forces 
resulting from the great increase of specific surface. Such a dis- 
persion could lead to a more rapid digestion of the fats, thus 
materially aiding the transformation of fats to carbohydrates and 
the accumulation of energy for germination. The importance of 
making the fats more capable of transformation to carbohydrates 
should not be overlooked. It is also probable that this dispersion 
reaches a degree of division where it could aid in the translocation 
of fats as such. Thus highly dispersed fatty material would be 
carried through the cell walls at points of protoplasmic connection. 
