1921} PACK—JUNIPERUS 51 
the seedlings gradually attained the ratio 1:1. This rise in the 
respiratory quotient was probably due to the oxidation of carbo- 
hydrates and the more intense respiration of the seedlings. 
Table XVIII gives the results of intramolecular respiration. 
The method used was that of Nicotas (26). The point to be 
noted here is the low 1/N ratio (the intramolecular or anaerobic 
respiration divided by the normal respiration) for the seedlings. 
Peroxidase was more generally present than oxidase. Quanti- 
tative oxidase activity determinations were made with the Bunzel 
apparatus. These results showed that there was no appreciable 
increase of oxidase activity until after germination. - 
TABLE XVII 
INTRAMOLECULAR RESPIRATION OF JUNIPER SEEDS, NO. 10, AT 25°C. (7.0C. VOLUME) 
Condition of seeds Weight Days (Percentage CO.| 1/N ratio 
After: 30 dave ats? Co AG 0.030 3 1.70 
After oo days at 5°C......... 0.028 3 1.67 0.43 
After roo days at 5°C...... 0.028 3 1.66 phe 
After 130 days at SC a 0.099 I 0.95 o.10 
The results of catalase determinations are given in table IX, | 
which gives the average of a great number of experiments. It was 
found that (1) when seeds were placed under ordinary germination 
conditions at 5° C. the increase of catalase activity gave a measure 
of the after-ripening; (2) the gain in catalase activity above that 
of air-dry seeds was greatest at 5° C. in a germinator; (3) the gain 
at the other temperatures was slow at best; and (4) seeds soon 
lose their catalase activity when in a germinator at temperatures 
above 25°C. The precautions used in the catalase determinations 
have been stated. 
Crocker (6) speaks of the rise in vigor of seeds, as shown by 
their resistance to fungal attack, during after-ripening. The 
juniper seed is protected against fungi before germination by the 
heavy lignin coat. It was found that juniper seeds which had not 
been after-ripened soon succumbed to fungal growths with the 
removal of the coats. After-ripened juniper seeds, however, 
when freed from the coats, withstood dense fungal growths. 
