92 BOTANICAL GAZETTE [FEBRUARY 
of the green leaf were completely filled with starch, which was 
present in larger grains than it was in the mottled leaves. The 
masses of starch here appeared to be about five times the diameter 
of the masses in the mottled leaves. At noon, a healthy plant 
which had the lower leaves mottled was placed in the dark. Imme- 
diately one-third of the tip end of one of the green leaves was cut 
off, then in 6 hours another one-third was cut off, and at the end of 
18 hours the remainder of the leaf was removed. The same was 
done with the mottled leaf. 
Table III shows that the translocation power of the leaf was 
still active, and proves that diastase was not inhibited by oxidizing 
enzymes, as was believed by Woops (18) in the case of mosaic 
leaf of tobacco. 
TABLE III 
TRANSLOCATION OF STARCH 
Leaf Placed in darkness After 6 hours After 18 hours 
Green, 22s ee Much Medium Little 
Mottled .| Fair amount Less starch Minute traces 
Tests for iron in the chloroplasts were made with potassium 
ferrocyanide. Both the green and the mottled leaves had iron in 
their chloroplasts. The chloroplasts which were present in the 
mottled leaf were colored about as deeply as those in the green 
leaf. From the blue tint which was produced in the leaves, the 
green ones appeared to have more iron than the mottled ones. 
From the macrochemical results which follow, it is evident that 
some of the iron in the leaves is ‘‘masked.”’ 
In comparing the amount of ammonium magnesium phosphate 
crystals which were formed in the two leaves on the addition of 
sodium ammonium phosphate, there appeared to be slightly more 
crystals formed in the cells of the mottled leaf blade. In both green 
and mottled leaves less magnesium was found in the upper part of 
the petiole than in the part of the petiole nearest the stem. The 
petioles of young green leaves had about the same amount of 
magnesium as the petioles of strongly mottled leaves. In the 
petioles about one crystal of ammonium magnesium phosphate 
