106 BOTANICAL GAZETTE — [FEBRUARY 
nitrogen per 100 gm. of wet weight show that the mottled leaves 
had much less of the albuminoid ammonia. 
Distillation of the leaves with Na,CO, was also undertaken. 
Twenty-five gm. of the leaves was distilled in a flask with 2 gm. 
TABLE XVI 
ALBUMINOID AMMONIA 
Sampl Sampl Sampl yermortiny 
am pie ample mple nitrogen 
Leaf no. I no. TT oi II Average per roo gm. 
dry weight 
oe er er ey 0.05 0.120 0.06. 0.079 1.18 
Mottled FEES Agee ae 0.019 0.035 0.019 0.024 °.4I 
Mottled 6x... 3.434 ss 0.018 0.024 0.013 0.018 0.29 
of Na,CO, until 60occ. of distillate was collected. An aliquot 
portion of it was Nesslerized and calculated as grams of nitrogen 
per 100 gm. of wet weight. Table XVII shows that the mottled 
leaves were lower in the amount of ammonia set free. 
TABLE XVII 
AMMONIA FROM DISTILLATION witH Na,CO,; 
Sample Sampl Sampl pe ioseebiy 
am itrogen 
Leaf mo noi =| no dit | Average | per roo gm. 
dry weight 
Greet. ree 0.012 0.0044 0.019 0.012 o.18 
Mottled Aion os ssssces; ©.0073 0.0031 0.0095 0.0066 O.11 
Motta Se... 0.0056 0.0040 0.0064 0.0053 0.085 
Nitrites were determined by the method as given by DAVISSON 
(rz). Attempts were made to determine the amount of nitrite by 
colorimetric methods (29), but the presence of anthocyanins which 
could not be precipitated out with lead subacetate interfered. 
TABLE XVIII 
NITROGEN AS NITRITE 
Sampl S le $ ee 
mple am ample nitrogen 
Leaf no. I no. bat no. III Average | per roo gm. 
dry weight 
ICON es. Seu es None None None None None 
AOU cs a cent ©.001,12 | 0.001,32 | 0,001,42 | 0.001,29 | 0.021,5 
