1921] SCHERTZ—MOTTLING IIt 
was made in g5 per cent alcohol at the temperature of the boiling 
solvent for 20 hours, and then for 4 hours in ether at the temperature 
of the boiling ether. The residue was dried, weighed, and called 
F;, the ether-alcohol extract was F, and F, combined. The ether- 
alcohol extract was evaporated to dryness in a Freas vacuum oven 
at 70°C., weighed, and the portion of it which was soluble in 
anhydrous ether at room temperature was called F,, and the weighed 
residue F,. Aliquot parts of the three fractions were analyzed 
and results were calculated to grams of N or P per 100 gm. of fresh 
leaves. 
TABLE XXV 
PROPORTIONS OF VARIOUS FRACTIONS IN GREEN AND MOTTLED LEAVES, WEIGHTS 
OF Fy, F2, AND F; 
Leaf Sample ne.I | Sample no. II | Sample no. Tit Average 
ie ee vic chee: 4.41 4.78 4.60 4.60 
Orie Fe ies 1.39 1.23 1.38 1.33 
Be eae cs 0.63 0.65 °.69 0.66 
Total dry weight........ 6.43 6.66 6.67 6.590 
PAM A, Be css. 3.66 3-94 3-74 3.78 
Mottled 4, Fo. ooo fo 1.61 1.46 1.68 1.58 
MOU A, Fyici as. cc 0.16 ©.13 °.10 0.13 
Total dry weight. ....... 5-43 5-53 5.52 5-49 
MOM AP ac 4.53 4:77 4.31 4-54 
Mottled B, oe t.56 1.55 1.38 1.49 
Mottled B, | PR USER Pp 0.20 0.40 0.39 0.33 
Total dry weight........ 6.28 6.72 6.08 6.36 
In comparing the weights of F,, it is seen that there was a 
decrease in weight as the leaves mottled, while the weight of F, 
increased and F, greatly decreased. The decrease in weight of 
F; is accounted for by the fact that the carbohydrate synthesis 
is considerably reduced, as was shown in table XIX, where the 
carbohydrates of the leaf were compared in the morning and in the 
evening. The increase of the weight of F, can be accounted for by 
the accumulation of salts in the older leaf. “The results of F, 
showed that the sulphatides, phosphatides, nucleo-proteins, fats, 
etc., were greatly reduced in the mottled leaves, and many of these 
were nitrogen complexes. 
