128 BOTANICAL GAZETTE [FEBRUARY 
14. Mottling of the leaves greatly lessened the carbohydrate 
output. 
15. The catalase activity of the leaves was very greatly reduced 
as the leaves mottled. 
16. The dry weight of the mottled leaves was less and the ash 
greater than that of healthy green leaves. 
17. In mottled leaves the weight of F, and of F,; was less, while 
that of F, was more than that of the green leaves. 
18. Protein nitrogen decreased in all of the three fractions as 
the leaves mottled, while phosphates decreased in F;, increased in 
F,, and almost disappeared in F,. 
19. The amount of chlorophyll (@ and 6) was about one-fifth 
of that of lilac leaves; the proportion of a to b was the same as in 
other plants (lilac). 
20. The carotin and xanthophyll content greatly increased as 
the leaves mottled. 
21. Bacteria were found within the cells of the mottled leaves, 
but it is not known whether they bear a causal relation to mottling 
or not. 
Great indebtedness is due to Professor WILLIAM CROCKER 
for his keen analytical interest and advice, and to Dr. S. H. ECKER- 
son for her untiring interest and suggestions on the problem. 
Bureau or Piant INDUSTRY 
Wasarncton, D.C. 
LITERATURE CITED 
1. APPLEMAN, C. O., Some observations on catalase. Bor. Gaz. 50:182-192. 
IgIo. 
. ARTARI, ALEXANDER, Uber die Bildung des ae durch griine 
Algen. Ber. Deutsch. Bot. Gessells. 20: 201-207. 
3. Aso, K., and Sex1ne, T., Uber das vorkommen von paeikae in Pflanzen. 
Beih. Bot. Centralbl. 32: ieee. 1914. 
. Boncguet, P. A., Presence of nitrites and ammonia in diseased plants. 
Its significance with regard to crop rotation and soil depletion. Jour. 
Amer. Chem. Soc. 38:2572-2576. 1916. 
. Boncquet, P. A., and BoncquEt, Mary, Presence of nitrites and ammonia 
in diseased plants. Oxidases and diastases; their relation to the disturb- 
ance. Jour. Amer. Chem. Soc. 39: 2088-2093. 1917. 
N 
> 
wm 
