256 BOTANICAL GAZETTE [APRIL 
for long periods and to a rather high degree, especially those of 
Verbascum Thapsus and Nicotiana Tabacum. Attention should be 
called to low germination of newly harvested seeds of Oenothera 
biennis, Daucus Carota, and Rumex crispus. 
Tests for evidence of 
TABLE III 
INCUBATED INCUBATED INCUBATED INCUBATED 
9-18-15 TO 10-28-15 TO 5-30-16 TO 6-20-18 TO 
II-5-15 6-6-16 6-28-18 
SEEDS 
Lae | eT ae Oe tte | OS | te | 
Collected in 1914 
1. Nicotiana hybrid...| gt 56 87 65 87 60 84 45 
3. Nicotiana affinis....| 79 Oe, 87 45 74 43 82 19 
8. Nicotiana hybrid....} 48 I 58 5 74 35 67 13 
13. Nicotiana hybrid.. . 84 ° 89 2 89 6 80 ° 
22. Nicotiana hybrid....| 55 ° 73 ° 61 re) 55 ) 
55. Verbascum Thapsus.| 81 I 80 ° 87 ° 53 ° 
61. Daucus Catota:... - 63 19 78 10 7I 20 32 7 
66. Oenothera biennis...| 4 2 76 10 51 4 fe) ° 
68. Rumex crispus...... 39 8 88 18 75 31 60 I 
92. Nicotiana Tabacum.| 77 Babe ee ray go 4 30 ° 
96. Daucus Carota..... I ° 52 Oe Ai es plane 
Collected in 1915 
97. Verbascum Thapsus.| 94 ° 94 re) 82 St aes 
98. Oenothera re. ne 2 ° 48 ° 61 SA ye aes 
99. Rumex crispus...... 6 ° 82 4 80 40 66 ° 
roo. Verbascum Thapsus.} 74 I 73 ° 39 ° 60 ° 
ror. Rumex crispus..... I ° 94 ° g2 2 30 ° 
ro2, Daucus Carota. oo i 45 I 44 Po ae 
103. Oenothera biennis.,.|......}...... 63 ° 61 ro eee re 
TO4. MUIOes Cr. dt 04 I 79 a ee meena AC esse 
105. ari Suse seed- 
Ee ee EE Panay Perec Gan) FOR SOUS Neen 75 ca ea angie CAR ee 
III Cienactiont in 
Rs ee a er Ob 75 ree I te ge r a 
112. Honduras es sori: Oa eaaige (aera lia (ear re 49 Ofc eee 
¥i3. Cuban topatee. ea ee Se 81 opty Bek y Cas ea 
117 Pennsylvania Ha- 
Nal aia eeelenes fie he 100 Oe de aie es 
118. Ohios seotlent set ae Ue omy oor vere ed 86 Pe ee se he 
a period of after-ripening in Verbascum Thapsus were quite nega- 
tive. Newly harvested seeds of V. Thapsus germinate above 9o 
per cent in light and only about 2 per cent in darkness. Tests of 
still older seeds indicate that they retain light sensitiveness as long 
as they are viable. 
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