1921] GARDNER—GERMINATION ; 285 
2. Abrasion and removal of coats (ovary walls) of Rumex crispus 
seeds promoted their germination in darkness. 
3. Treatment of seeds of Rumex crispus and Oenothera biennis 
with concentrated sulphuric acid caused an increase in the per- 
centage of germination in darkness. 
4. No reciprocal relation between the effects of light and temper- 
ature was found. 
5. Light was not necessary for the absorption of sufficient 
water for germination. 
6. Injection of water did not yield increased germination in 
darkness. 
7- Almost all kinds of single electrolytes, regardless of the nature 
of the ions, seemed to promote germination of seeds of Oenothera 
biennis, Nicotiana Tabacum, and Verbascum Thapsus in darkness. 
8. Embryos of seeds incubated in light became more acid than 
those incubated in darkness. 
9. Light seemed to activate lipolytic enzymes which hydrolyzed 
a to fatty acids. 
10. The germination of seeds of Rumex crispus in darkness was 
promoted (increased) by hot water treatment, abrasion, treatment 
with concentrated sulphuric acid, increased oxygen pressure, 
fluctuating temperatures, and soaking in solutions of hydrochloric 
acid, sodium sulphocyanate, and hydrogen peroxide. 
11. The germination of seeds of Nicotiana Tabacum in darkness 
was promoted by soaking in solutions of hydrochloric acid, sodium 
sulphocyanate, and hydrogen peroxide, as well as by the use of many 
single electrolytes as substrata. 
12. The germination of seeds of Verbascum Thapsus in darkness 
was promoted by the action of light, fluctuation of temperature 
during incubation, alternating high and low temperatures, soil, and 
many single electrolytes as substrata. 
13. The germination of seeds of Oenothera biennis in darkness 
was promoted during certain seasons by hot water treatment, 
sulphuric acid, preliminary incubation at low temperature, incuba- 
tion in alternating high and low temperatures, and single electro- 
lytes as substrata. 
