348 BOTANICAL GAZETTE [MAY 
weighings were made as before. The desiccators in these experi- 
ments were kept in a refrigeration room where the temperature 
remained quite constant at about 34°F. This low temperature 
Ti=37.45. 
AF"04.9U 
£45 
5 SOY eae 
T2—52.15 
Fic. 3.—Loss of moisture from detached buds exposed to an atmosphere inclosed over sulphuric acid of 
different concentrations; A, Assiniboine; 7, Tonka; numbers indicate concentration of acids; C, distilled 
water check. 
was chosen as a precautionary measure against the possibility of 
the occurrence of any growth changes. Fig. 3 shows that the loss 
is almost uniform in both hardy and semihardy buds in every case 
