420 BOTANICAL GAZETTE [JUNE 
dark room in which these seedlings were grown was 21°-23°C., 
which probably accounts for the greater length of coleoptile and 
root as compared with seedlings of the same age used in study- 
ing the carbohydrates. In sample A the air-dry wheat was finely 
ground in a food chopper, and in B and C the seedlings were 
cut up into small pieces with scissors immediately after removal 
from the germinating dishes. In all cases the material was 
preserved in 7o per cent alcohol. After extraction in hot alcohol 
TABLE V 
ANALYSIS OF MARQUIS WHEAT (1917) FOR AMINO NITROGEN CONTENT 
vice ge 
Sample Percentage Percentage luble 
. dry weight fev ien 
A A cape 
Ee ee iyiew eet Sasi eh 10.78 CG ee eee bee oye ee 
Soluble siticaee Ware Peer eale ees Liles eee ees 6164-8 
em er as 0.0275 16.76 
for six hours the material was ground in a mortar and a hot 
water extract made. As the presence of so much colloidal mate- 
rial (starch and protein) made it impossible to’ separate at all 
accurately the water from the solid matter, the entire mass Was 
made up to 75 per cent alcohol, and all colloidal material pre- 
cipitated by shaking with NaCl. The material was then filtered 
and the filtrate combined with the alcohol extracts and con- 
densed in vacuo at 60°-70°C. to approximately socc. This 
amount was then made up to volume (100 cc.) with distilled water. 
Determinations were made on this material for total nitrogen and 
