422 BOTANICAL GAZETTE [JUNE 
3. Reducing sugar (probably all glucose) appears in the embryo 
after 18 hours in the germinator. It is first found in the coleo- 
rhiza, but soon afterwards appears in considerable quantity in 
all parts of the seedling, especially in the zone of root hairs and 
coleoptile. 
4. During the germination period studied the increase in length 
of epithelial cells averaged 150 per cent. 
5. Peroxidase and catalase are present in all parts of the grain 
both before and during germination. The amount of catalase 
present increases during the first seven days at a rate corresponding 
to the rate of increase in the respiratory activity. 
6. During germination the protein content of the endosperm, 
except for that of the aleurone layer, decreases greatly. 
7. Microchemical analyses show the presence of amino acids in 
the ungerminated grain and their increase in amount during 
germination. Microchemical analyses fail to indicate any amino 
nitrogen until the fourth day of germination. Asparagine is the 
only form that can then be so identified. This appears only in 
the root and coleoptile, accumulating in the latter in considerable 
quantity. 
Microchemical tests 
Pectic substances.—Ruthenium red, red color; methylene blue, 
violet color. 
Lignin.—Phloroglucin and HCl, violet red color obtained with- 
out heating. 
Suberin.—Insoluble in cold 50 per cent chromic acid. 
Starch.—lodine-potassium iodide, blue color. 
Dextrin.—Amylo-dextrin, iodine-potassium iodide, red color; 
dextrin, precipitation of cuprous oxide upon long heating with 
Fliickiger’s reagent (see under fructose). 
Fructose —Fliickiger’s reaction; copper tartrate dissolved in 
15-20 per cent NaOH, red precipitate obtained at once without 
heating. 
Glucose.—Fliickiger’s reaction; red precipitate of cuprous oxide 
on heating 1-2 minutes; osazone crystals with phenyl-hydrazine 
reaction. 
