Ate 
ii natalia ctenitmenn™ 
1921] PACK—JUNIPER SEEDS 141 
seedlings of the sunflower, and found that glutamin and asparagin 
increased during germination. PALLADIN (18) in 1896 stated that 
an increase of the proteins indigestible in gastric juice (nearly 
proportional to the amount of nucleo-proteins) accompanied the 
germination of wheat in darkness. ZALIESKI (22) in rg11 reported 
the general appearance of nucleo-protein in plant tissues. He also 
found an increase of nucleo-protein with the germination of wheat 
and corn seeds. 
Investigation 
CULTURE METHODS 
After the seeds had been prepared, as described in an earlier 
paper (17), they were placed on moist filter paper in Petri dishes 
and subjected to a temperature of about 5°C. in darkness for after- 
ripening and germination. Distilled water was added at intervals 
to keep them moist. The changes occurring had to do with the 
reserve material already in the seed. The after-ripened seeds and 
seedlings were kept under these conditions until being prepared for 
analysis. Analyses were made at three different stages: dry seeds, 
after-ripened seeds, and late seedling development. 
Dry sEEps.—The hard coats were removed from the dried seeds 
before preparing them for analysis. In this, as well as in the 
following stages, the embryo and endosperm (or more exactly, the 
nucellus, megaspore membrane, and all parts surrounded by these 
two structures) were the parts analyzed. 
AFTER-RIPENED SEEDS.—The seeds were found to require 100 
days’ storage in a germinator at about 5°C. for after-ripening (or 
to become ready for immediate germination). The seed at this 
particular period has already split open the hard coat, and the 
hypocotyl is breaking through the nucellus. It was at this time 
that the after-ripened seeds were removed from the hard coats and 
prepared for the analysis. 
DEVELOPED SEEDLINGS.—It required 35 days at 5°C. for the 
after-ripened seeds to become developed seedlings, which were 
between 3 and 4cm. long. The cotyledons were extended and 
free from the old endosperm, nucellus, megaspore membrane, etc. 
These latter structures were collected and put with the seedlings 
