144 BOTANICAL GAZETTE [SEPTEMBER 
fraction. The percentage of phosphatide was estimated from the 
lipoid; P times the factor 25.77. Wuj’s iodine solution was used 
in the determination, of the iodine number. No direct nitrogen 
determination was made on the lipoid fraction. The percentage 
of nitrogen given was found by subtracting the extractive and 
protein nitrogen (tables III and IV) from the total nitrogen given 
in table I. Table II also gives the percentage of oxygen taken up 
by the lipoid material under artificial oxidation. 
TABLE III 
EXTRACTIVES (F2) 
SEEDLINGS 
PERCENTAGES OF TOTAL 
DRY WEIGHT 
a b c a b c ‘ b c 
Total extractives. . O.G8 PP O.s0) fis 94 las be |. (84002 [94. §0];..-- 
Total gens 6:13 | @.2¢ 1.0 $0125 f 220 < ; 
nia nitrogen. .| 0.0004] 0.0004]... .| 0.0004] 0.0003}....| O.CO0I]..... es 
Amino acid nitrogen.| 0.04 | 0.05 |o.04| 0.27 | 0.25 |o.30| 0.92 | 0.95/0.93 
Reducing sugars after] 
hydrolysis........ 1.27 %.30. {%.25| 1-66 1.80. |1.88] 7.49 7.4317-55 
Direct reducing 
sugars........... Traces fe ceka ve SE OOF a. O108)-2:5395° | 3: 3418.37 
Reducing sugars 
after removal of 
NING ois es bees RYACRS be pase bss OG. Pe Ee ade cates es oe 
Pentose reaction. ape Pee ve ST RAPKORIS 3. 2) - ee ia aed Barenas, 
marked . 
Unaccounted for ma- 
terial (organic 
WON is 2 atin ha ee pee ed Adsseass PO Ey ae ee cane 
Table III gives the extractives as percentage of total dry 
substance. The total nitrogen was determined by the Bock and 
BENEDICT (1) modification of the Fotrn-FarMER (5) procedure. 
Ammonia nitrogen was determined by the same procedure after 
aerating under diminished pressure a large part of the extraction 
solution made slightly alkaline and collecting the ammonia in 
ilute HCl. The undistilled material was neutralized with acetic 
acid, concentrated, and used for van Slyke amino nitrogen deter- 
minations. A third portion was used for the sugar determinations. 
The tannins were removed with pure casein. Both after-ripened 
seeds and seedlings gave indications of pentose. 
