1921] PACK—JUNIPER SEEDS 145 
The proteins, polysaccharides, etc., are given in table IV as 
percentages of total dry substance. The protein nitrogen, which 
is stated as percentage of protein, was determined by the KJet- 
DAHL procedure. Polysaccharides were determined by the Mon- 
SON-WALKER and BERTRAND method. This material gave very 
marked pentose reactions. The cellulose was not determined 
as such, 
TABLE IV 
PROTEINS, POLYSACCHARIDES, ETC. (F3) 
Dry SEEDS AFTER-RIPENED SEEDS SEEDLINGS 
PERCENTAGES OF TOTAL DRY WEIGHT 
a b a b c a b 
ela pte eee etc. .} 30:73} 30.00) 40.83 | 40.43) .252 2. 54-25 | 54-40 
Total proteins (F; eo eee 34.21] ..34.10 — os 27 OSs ys 22.32: 1:33.90 
Total As sugars oan 0.0 0.0 0.23] ©.19] 14.91 | 14.79 
Indications of pentoses....... 6:0 106.0 Seco Nb a Pala ne CY bt 
marked 
Discussion 
These results force upon one’s attention the great constructive 
changes as compared with the destructive changes. The major 
fractions seem to be well accounted for. Such a condition can only 
be understood when one considers that these results deal with a 
seed that requires long continued after-ripening and germination 
at a very low temperature. Although the seed material was kept 
at a temperature of about 5°C., the constructive metabolism went 
on at a rapid rate. The digestion of storage ae and gplscomilg was 
accompanied by the synthesis of many f 
pounds. The rate and extent to which these changes were carried 
on even at 5°C. prove the power and efficiency of enzyme action. 
This low temperature, by retarding respiration, reduced the com- 
bustion of materials to a minimum, and thereby favored the 
accumulation of formative materials in the cells. This accumu- 
lation of cell building and cell active materials, together with the 
culmination of enzymes, probably leads to the after-ripening of 
dormant organs. 
The lipoids decreased 9.7 per cent during after-ripening, and 
32 per cent during the seedling development. It will be seen that 
