1921] HOERNER—GERMINATION 175 
oped on Improved Ligowa Oats (Minn. 281), one on A. sterilis, 
two on Improved Ligowa Oats (Minn. 281), followed by one 
generation on A. sterilis. 
e) Rust collected on A. sativa at Lynchburg, Virginia, was used 
as the original inoculum. Subsequent inoculations resulted in five 
spore generations being developed on Improved Ligowa Oats (Minn. 
281), one on A. fatua, followed by two generations on Improved 
Ligowa Oats (Minn. 281). : 
f) Rusted specimens of A. fatua were collected at San Diego, 
California. The spores were used as the original inoculum. Sub- 
sequent inoculations resulted in thirteen successive spore genera- 
tions being developed on Improved Ligowa Oats (Minn. 281). 
g) Rust collected on A. sativa at Tallulah, Louisiana, was 
used as the original inoculum. Subsequent inoculations resulted 
in thirteen successive spore generations being developed on 
Improved Ligowa Oats (Minn. 281). 
Heavily rusted leaves were cut from plants in each series and 
placed in Petri dishes. Germination tests showed the spores from 
each series to be viable when removed from the greenhouse. The 
following environmental conditions were provided: 
A. Petri dishes were placed outside and protected by Pome 
with about one foot of leaves and snow. 
B. Petri dishes were placed outside and not afforded any 
protection. 
C. Petri dishes were wrapped in heavy ets paper and 
placed i in a dark cabinet drawer, indoors. 
D. Petri dishes were placed indoors fully exposed to sunlight. 
Material from series a, b, f, and g was subjected to environment 
A; from series a, e, f, and g to environment B; from series } and g 
to environment C; from series c and g to environment D. Series 
a, c, e, f, and g under all of the environmental conditions and series 
g under environments A, B, and D gave no positive germination 
tests. The germination tests from series 5 under all environments 
provided, of series d under environment A, and of series g under 
environment C were all positive. The temperature range for 
environments out-of-doors was between —27°F. and 42°F. inclu- 
sive, while for the indoors environments it was between 29° F. and 
