270 BOTANICAL GAZETTE [NOVEMBER 
MICROTOME SECTIONS.—These were made in order to show the 
morbid histology in comparison with the normal histology.: Because 
of the oil content of the nut, ether was found to be the best killing 
and fixing agent. The ether was replaced after three days with 
chloroform, and the ordinary schedule for imbedding with this 
reagent followed (2). After sectioning it was found that the oil 
content had not been sufficiently lessened, and that without its 
removal a distinct view of the structures could not be secured. To 
obviate the difficulty the sections were fixed to the slide, treated 
with xylol, xylol and absolute alochol, absolute alcohol, and then 
flooded four or five minutes with ether. The slide was held 
horizontally between thumb and finger, and the dissolved fats 
collected on the under side of the slide, from which they were wiped 
off before placing the slide in 95 per cent alcohol. ‘The slide was 
kept in each of the 95, 85, and 70 per cent alcohols for about five . 
minutes, and then flooded with Pianese IIIb for 15 minutes (32), 
washed with distilled water, 70, 85, and acidulated 95 per cent 
alcohol, 95, 100 per cent alcohol, 100 per cent alcohol and xylol, 
xylol, and mounted in balsam. The stain shows the host tissue 
in red and the fungus in green. 
Two of the fungi produced pycnidia which were sectioned for 
study. These were taken from cultures on cornmeal agar, killed 
and fixed with chromacetic fixing fluid, and stained with Bismark 
brown, following the usual schedule for this stain. The pycnidia 
were removed from the culture on a strip of agar, usually about 
1 X2X4 mm. in size, which remained intact throughout the process 
and served well in the orientation of the specimens in the imbedding 
dish. 
FREEHAND SECTIONS.—It was occasionally found necessary 
to stain razor sections made for the preliminary study of the dis- 
eased tissue. The sections were cut as thin as possible and placed 
in a watch glass contained in a Petri dish. The watch glass was 
then filled with ether and the Petri dish closed. When the ether 
had evaporated, 95 per cent alcohol was poured over the sections and 
allowed to stand for ten or fifteen minutes. This was followed 
with 85, 70, and 50 per cent alcohols for five minutes each. The 
_ sections were then transferred to slides prepared with albumen 
