286 BOTANICAL GAZETTE NOVEMBER 
aerial mycelium dense, white; soluble pigment none; enzymatic 
zone three to four times the diameter of thallus. 
6. Cornmeal agar: growth dense but zonated, Pale Pinkish 
Buff; aerial mycelium powdery; soluble pigment none. 
7. Egg albumin agar: growth thin, conspicuously zonated, 
Pale Pinkish Buff; aerial mycelium powdery, Pale Pinkish Buff; 
soluble pigment none. 
8. Nut plugs: growth vigorous, Pale Pinkish Buff; aerial 
mycelium powdery, white; medium not completely destroyed, 
but much shrunken and blackened. 
9. Autoclaved rice: growth vigorous, Pale Pinkish Buff; aerial 
mycelium 2cm.; almost completely destroyed in sixty days. 
1o. Potato plugs: growth vigorous, crumpled, Pale Pinkish 
Buff; aerial mycelium abundant, at first white, later Pale Pinkish 
Buff; medium with no change in seis much reduced in size in 
two months. 
11. Carrot plugs: growth at first slow, appearing after five 
days, crumpled and dense, Pale Pinkish Buff; aerial mycelium 
powdery, at first white, later Pale Pinkish Buff; medium darkened 
near the growth, no change in color in other regions, much shrunken. 
12. Brazil nut bouillon: growth surface pellicle, snow-white; 
aerial mycelium white, powdery; medium somewhat clarified. 
13. Nut strips: growth slight on strip above water, and none on 
strip in water; no growth on surface of water. : 
BIOCHEMICAL FEATURES.—The proteolytic enzyme which makes 
the halo in Brazil nut agar plates was the only one studied, but the 
growth reactions in different media were taken to indicate the 
probable production of several other enzymes, diastase and inver- 
tase especially. The proteolytic enzyme was isolated by precipita- 
tion, as previously described, and its proteolytic power tested by 
placing drops of a water solution of the dried precipitate on Brazil 
nut agar plates. Transparent areas the size of the drops developed 
in from two to three hours, depending upon the thickness of the 
agar plates. 
Taxonomy.—The organism is an Actinomyces which, according 
to WAKSMAN’S key, belongs in division B, ‘‘no soluble pigment 
produced on gelatin or other protein media,” and in section J, 
