288 BOTANICAL GAZETTE [NOVEMBER 
spores are oblong-elliptical, hyaline, guttulate, and measure 
5-7 X1.7-3.5 uw. The B spores are filiform, usually hook-shaped, 
hyaline, continuous, and measure from 17—24.5 X2-3.5 yp. 
CULTURE CHARACTERS.—The fungus grew well on all media 
used. On cornmeal agar the thallus was circular and without » 
zonations. A loose aerial mycelium covered the entire thallus, 
and numerous pycnidia varying in size were scattered over the 
surface of the plate. The pycnidia appeared simultaneously with 
the brown color, which was usually noticed after five or six days. 
On Brazil nut agar the growth was similar to that on cornmeal 
agar. The clear halo formed in the agar plate barely exceeded 
the size of the thallus. On autoclaved rice a brown or smoky color, 
due to the mycelial growth, was noticeable, but no color change 
occurred in the medium. Nut plugs were soon covered with a 
brown mycelium which later became almost black. The surface 
was soon covered with black, wartlike pycnidia, and the entire 
_ mass when cut through suggested a dried sponge. The odor was 
similar to that of very rancid nuts. The fungus made no growth 
on nut strips above the water, but a dense mass of mycelium, filled 
with black strands, developed on the strips in the water. These 
strips retained their form, but the cessation of mycelial growth, 
which occurred between ten and fifteen days after inoculation, 
. marked the time of nutrient exhaustion. 
Taxonomy.—The fungus is a typical Phomopsis which 
approaches P. aucubicola Grove more nearly than any other 
described species. A spores are shorter, and this, coupled with the 
fact that it occurs on an unrelated host, necessitates describing it 
as new. 
Phomopsis bertholletianum, n. sp.—Pycnidium dark brown, 
mammiform, with wartlike protuberance, irregular in shape and 
size, varying from o.1 to 1.0; conidiophores filiform, hyaline, 
continuous, 15-20 yw long, often indistinguishable from B spores. 
A and B spores present, A spores oblong-elliptical, hyaline, guttu- 
late, 5-7X1.7-3-5@; B spores filiform, usually hook-shaped, 
hyaline, continuous, 17-24.5 X 2-3.5 um. 
Hasitat.—Parasitic on kernels of Brazil nuts. 
