Io BOTANICAL GAZETTE [JANUARY 
included, were obtained in a like experiment with Laelia-Caitleya 
hybrid no. 2. . 
_ The degree of development represented by the numbers 1 to 
8 is as follows: (x) dark green seedlings, most of these with two leaves 
and a few showing roots; (2) seedlings the same as no. 1, but light 
green; (3) seedlings light green, most of them with one leaf and a 
few showing two leaves; (4) seedlings light green, with only one 
leaf and that leaf short; (5) about 50 per cent of embryos showing 
leaf point; (6) embryos just showing a depression in meristem 
region; (7) advanced spherule stage; (8) smaller spherule stage. 
The data in table I show that fructose is more favorable for 
growth of the embryos than glucose. This is apparent not only 
in the percentage showing leaves, but in the general appearance 
of the cultures. The embryos in the glucose cultures were whitish 
or yellowish in color. On the other hand, the fructose cultures 
were dark green. A more striking difference was noted on March 
27, when in the glucose cultures the embryos were still yellowish 
and had shown no appreciable gain since January 27. The fructose 
cultures, on the other hand, had progressed and were still more 
markedly superior to the glucose cultures than on January 27. 
Fig. 1 shows the fructose culture and the nutrient solution culture 
minus sugar. 
The addition of a plant extract to the glucose cultures has a 
marked effect on growth and chlorophyll development. In each 
case the percentage of germination is higher than with glucose 
alone, and the ranking of glucose-containing cultures on March 27 
indicates that those with yeast or wheat extract rank with the 
best cultures, that with beet extract ranks in the third group, and 
the cultures with glucose alone fall in the fourth group. The addi- 
tion of plant extract to the fructose-containing media is practically 
without any beneficial effect. 
The loss or lack of development when glucose is supplied in 
the nutrient solution has been noted by Mazé and PERRIER (9) 
for corn, and by SERVETTAz (10) in nutrition experiments with 
moss. In the case of orchid embryos the chlorophyll makes its 
appearance only when the leaf is developing, and then generally 
only in the leaf. Even then the leaves are only of a light green 
a a6 
