16 BOTANICAL GAZETTE [JANUARY 
germinated and possessed one or two leaves. On the cultures not 
inoculated, the embryos lacked chlorophyll, and not one had 
produced even the leaf point. Most of the embryos exhibited the 
depression at the meristem region. Two months later some of 
the embryos, although still lacking chlorophyll, had formed the 
leaf point; while in the inoculated cultures the seedlings had 
two and three leaves and some had already produced roots (fig. 2). 
The results appeared so unusual that the experiment was 
repeated with seeds of Laelia-Catileya hybrid no. 3, using solution 
B and also Ashby’s solution. The composition of the nutrient 
TABLE IV 
INFLUENCE OF Seated radicicola ON Laelia-Cattleya 
re S SOWN MARCH 14, 1921; 
NOTES TAKEN i Andon 10, 1921 
Average width 
Nutrient medium tn enleean 
solution Bev ee, 271 
Solution rhe 4% glusese 407 
Solution b-4270°% @iucdse i 6. is oe ics 814 
Sontion B--moculateds 0 0h. oe 1904 
Solution B+-o.1 glucose inoculated.............. 378 
Solution B+1.0% glucose inoculated............ 834 
AghDY'S MGtION 6 6 ee a 194 
Ashby’s solution+o.1% glucose... .............. 446 
Ashby’s solution+1.0% glucose................. 698 
Ashby’s solution+-inoculated...............--00- 164 
Ashby’s solution-+-o.1% glucose inoculated. ...... 307 
Ashby’s ana nat o% glucose inoculated....... 970 
G sition: C. superba Schomb. 4; C. Dormaniana Reichb. 3; 
iG, Wiceedaw Reichb. 4; L. purpurata Lindl, }. 
solution and the width of embryos are given in table IV. The 
favorable influence of Bacillus radicicola was noted only in the 
cultures having 1 per cent glucose. On solution B+1 per cent 
glucose the diameters of the embryos averaged the same for both 
the inoculated and the uninoculated seeds, but there was a striking 
difference in the color and the number with leaves. In the unin- 
oculated, 20 per cent of the embryos were showing the leaf point, 
but the embryos were whitish in color. In the inoculated, 50 pet 
cent of the embryos showed leaves, the leaf development was 
greater, and the embryos were green. 
