18 BOTANICAL GAZETTE [JANUARY 
and four without sugar. On August 20, 1921, notes were made 
on these cultures. In the sucrose cultures the seedlings had made 
a marked development. The largest seedlings had four and five 
leaves, some of the leaves being 2 cm. in length; while the roots of 
these seedlings, two or three in number, were 2-5 cm. in length 
(fig. 3). In the cultures lacking sugar the growth was less striking, 
the leaves being 2-4 mm. 
and the roots 2-10 mm. in 
length. 
Seedlings were trans- 
planted at the same time 
from D 23 to a compost of 
peat and sphagnum in or- 
dinary flower pots. These 
seedlings, on August 20, 
1921, showed leaves 5-7 
mm. and roots 1-2 cm. m 
length. These _ seedlings 
were better than _ those 
planted on solution B in 
the liter flasks, but not so 
good as the seedlings on 
solution B plus 2 per cent 
sucrose in the liter flasks. 
That better growth is 
possible in 150 cc. to 500 
Fic. 3.—Seedlings one year old on solution — Erlenmeyer flasks than 
B-+2 per cent sucrose; X?. in culture tubes was dem- 
onstrated repeatedly dur- 
ing these experiments. Erlenmeyer flasks of from 150 to 500 CC. 
capacity, containing the culture media left over after supplying 
the tubes with the requisite amount, were generally planted with 
the seeds that remained after the tubes were sown. In practically 
every instance germination took place sooner in the flasks than in the 
tubes. The probable explanation is that in the tube cultures the in- 
ward diffusion of carbon dioxide is impeded to a certain extent by the 
cotton plug, and, the volume of air in the tube being small, the carbon 
