232 BOTANICAL GAZETTE [MARCH 
substrate free from combined nitrogen, pure culture seedlings 
were planted in a similar medium lacking nitrates, both sets of 
seedlings being infected fromapure culture of the endophyte at plant- 
ing. These cultures were first grown in 1915, and no special pre- 
cautions were observed beyond 
the use of pure chemicals and 
freshly distilled water. The seed- 
lings not supplied with nitrate 
grew surprisingly well. They 
Fic. 3 Fic. 4 
Fics. 3, 4.—Fic. 3, control seedlings planted simultaneously with those in fig. 2 
in silica jelly nutrient containing combined nitrogen in form of KNO,; fig. 4, same 
edling as shown in right-hand tube of fig. 2, five and a half months old; shoot 
reached height of over 6 cm. 
were, on the average, healthier than the controls, a brighter 
green, and of quite as vigorous growth. The controls were 
supplied with potassium nitrate in the proportion of 0.5 gm. 
per liter. They showed no differentiating features and unfor- 
tunately were not photographed. It should perhaps be men- 
tioned that the cultures were grown in a small cold greenhouse 
away from the laboratory. Kjeldahl estimations of samples 
of the agar medium subsequently made yielded negative results. 
