406 BOTANICAL GAZETTE [may 
at the time of fusion. This method is highly recommended by its 
author to give very close checks, even though they are lower than 
those of the peroxide method. BENEDIcT attributes these high 
results in the peroxide method to silica, which is entirely lacking 
in his method. The writer experienced no trouble with silica in 
using the official method, for at no time was strong alkali allowed to 
stand in contact with glass without being neutralized. The ease 
with which the material was removed facilitated quick neutraliza- 
tion. There was never a weighable amount of silica found in the 
solution. 
Sulphate sulphur determinations 
The method of determining sulphate sulphur was the same as 
that used by Ames (1). Five grams of the dry material mixed with 
one per cent of hydrochloric acid were shaken in the mechanical 
shaker for three hours. The solution was filtered and an aliquot 
of 2.5 gm. taken. This was then treated with barium chloride 
and precipitation allowed to take place in the cold. After standing 
for at least forty-eight hours the sulphates were determined in the 
usualway. The averages of the determinations are given in table IT. 
TABLE II 
. Percentage | Percentage : 
5 Nf : Weight Total Total Nitrate 
se € Moisture Bas0, said ur oe po oo oem nitrogen 
Circleville, Missouri (5 gm.) 
eee 7.58 0.13 0.0179 | 0.387 0.01 2.41 o.O1 
ESS 7.59 ©.1320 | 0.0183 | 0.397 0.01 2.42 0.01 
Se ©.1246 0.0182 oY ae (eareree res Nata Ar rs 
Bie ellos otek: Oits64 0 O.O07E 1) 6. 855 cols. a aly 
pe ery AON aE 0.1262 0.0173 On976 bec anes ileee sens weir eee 
Sane Be 
Emporia, Kansas (1.25 gm.) 
: a 5.81 0.0348 | 0.0047] 0.405 0.013 B.GR |ecceesess 
Ea hee 5.82 | 0.0369 | 0.0050 | 0.424 | 0.012 2.31 Trace 
bg erence Se eee 0.0328 | ©.0045 b 6.380. foie es [e see ris ee 
Bey eel ea es ee 0.0529 -0045 O. B82 foe ee fe ceevees peter tetas 
A eo or 0.0371 0.0051 A et Core es Pe des 
eden! 
