1922] GARDNER & KENDRICK—TOMATO MOSAIC 471 
The possibility of the presence of the mosaic virus dried on the 
exterior of the seed coat was also taken into consideration. About 
four ounces of tomato seed collected from mosaic plants four months 
previously was washed in sterile water, and eight tomato plants 
were inoculated by wounding the stem near the growing tip with a 
needle, and rubbing the wounded area with cotton soaked in this 
wash water. No mosaic developed in these plants. In the light of 
this evidence there appears to be no indication that tomato mosaic 
is transmitted through the seed. 
Mosaic in perennial Solanaceous weeds 
HISTORICAL 
The susceptibility of certain perennial weeds to tobacco and 
tomato mosaic is highly significant in connection with the over- 
wintering of the virus. ALLARD (1) transmitted mosaic from tobacco 
to the perennial Solanum carolinense, and points out the possibility 
of the mosaic virus persisting over winter in the rootstocks of this 
weed. He states, however, that he had noted only one case of 
mosaic occurring naturally in S. carolinense, but recognizes the 
difficulty of detecting the disease in this weed because the symptoms 
may be very inconspicuous. He also found the mosaic which occurs 
commonly on the perennial Phytolacca decandra to be distinct from 
and unrelated to the tobacco mosaic. 
NISHIMURA (11) transmitted mosaic from tobacco to Physalis 
alkekengi. In his tests the Physalis plants developed no mosaic 
symptoms, but the juice expressed from the inoculated plants proved 
infectious to tobacco. This exotic species of Physalis is recorded as 
a perennial which is not hardy in the northern states. NisHIMURA also 
proved that a mosaic disease found on the perennial Solanum aculea- 
tissimum in Florida by R. A. HARPER was transmissible to tobacco. 
Recently CrAwrorp (6) in Iowa has reported successful cross 
inoculations from mosaic tomatoes to Physalis longifolia, a common 
weed of that region. He also found mosaic occurring in the field 
on that weed, and with the virus from the rootstocks made successful 
inoculations of tomato plants. He points out the probability of 
the mosaic virus overwintering in the rootstocks of Physalis longi- 
folia. This species has not been found in Indiana. 
