472 BOTANICAL GAZETTE [JUNE 
An example of the persistence of a mosaic virus in a perennial 
herbaceous host is afforded by the pokeweed mosaic studied by 
ALLARD (4). The work of CARSNER (5) on the weed hosts of the 
virus of the curly-top disease of sugar beets in California has been 
very suggestive in connection with the problem of overwintering of 
mosaic viruses. He has pointed out the probability that the curly- 
top virus may persist over winter in Erodium cicutarium, a winter 
annual. Recent work by Doo.irtte on the relation of Micrampelis 
lobata (7) and Asclepias syriaca (8) to cucurbit mosaic also has been 
suggestive. 
PERENNIAL SOLANACEOUS WEEDS IN INDIANA? 
The following Solanaceous perennials occur in Indiana: Lycium 
halimifolium Mill., Solanum dulcamara L., S. carolinense, Physalis 
lanceolata Michx., P. heterophylla Nees., P. subglabrata Mack. and 
Bush, and P. virginiana Mill. S. carolinense and the three species 
of Physalis, P. heterophylla, P. subglabrata, and P. virginiana, are 
weeds of common occurrence in and about cultivated fields. Of 
these, P. subglabrata and P. virginiana have been found to be by 
far the most abundant in the tomato regions, and most of the obser- 
vations have been made upon these species. These two species are 
not easily differentiated, and no consistent attempt has been made 
in this work to separate them. ‘The larger leaved P. subglabrata 
has appeared to be the more abundant of the two in central Indiana. 
Unless otherwise qualified, the term Physalis as used herein should 
be understood to refer to these two very similar species. 
CROSS INOCULATION TESTS 
Mosaic has been found occurring naturally in the field on 
Solanum carolinense, Physalis heterophylla, P. subglabrata (pl. XVID), 
and P. virginiana. On July 5, 1921, ten potted tomato plants in 
the greenhouse were inoculated by wounding the stem and rubbing 
the wounded area with cotton soaked in the juice from mosaic 
S. carolinense plants collected at Vincennes. By July 29 all had 
developed mosaic. None of the ten control plants, similarly 
treated except that distilled water was substituted for the juice 
?Cuartes C. Dram, state forester, very kindly furnished authoritative records 
concerning the Solanaceous flora of Indiana. 
