1922] LUPO—HY POXYLON 493 
which they increase their size, and at the time of the rounding out 
of the ascogonia there are four, sometimes more, large nuclei five 
or six times the size of the originals. As the ascogonia increase to 
the enormous odd shapes found in the more mature perithecia, the 
nuclei show great variation in size and number (fig. 11). During 
this stage they undergo rapid division without maintaining their 
size, and in the ascogonia which show evidences of budding out the 
ascogenous hyphae they are small and number sixteen or more ina 
section. Evidence for this interpretation of the nuclear program 
is gained also from the perithecial wall, because as it grows more 
mature in character these changes in size and number are unmis- 
takable. There is no evidence of fusion in any stage of the devel- 
opment of the ascogonia. No mitotic figures were visible, but in 
many cases the position of the two nuclei was such as to suggest 
late division. 
The ascogonia do not drop to the bottom of the perithecium 
before germinating. Instead the increase in size of the wall is 
toward the periphery of the stroma, and the ascogonia, although 
in the same actual position, seem to have dropped because of the 
expansion of the perithecial wall toward the surface. 
Ascogenous hyphae 
The material used was just at the stage when the ascogenous 
hyphae were beginning to bud out, and no detail about the forma- 
tion of these can be given. Great care was necessary not to con- 
fuse some of the stalk connections where separation was incom- 
plete with ascogenous hyphae. These could be recognized by their 
direction and evident connection with some part of the filament 
traced through serial sections. Legitimate cases of buds (figs. 12, 13) 
just formed were found, however, and although of course the nuclear 
situation, separation, and branching could not be determined, 
apparently the procedure would be the same as described for other 
forms where the nuclei migrate into the buds from the ascogonia, 
and further division takes place there. 
A supposition lacking evidence to support it is that the pa- 
raphyses described by DE Bary and Brown might be formed from 
these early ascogenous hyphae or branches of them instead of 
