IgIo] LIPMAN—LACK OF ANTAGONISM IN SALTS 43 
TABLE I 
Numbers in first column refer to c.c. of 0.35m solutions 
Culture solution finer Spear | celia De 
POO MOT oe A 3.08 
100 MgCl 
5 CaCl, ‘ puneon a ee B 2.59 
too MgCl, ? 
Io CaCl, \ Mei cate waa c 1.68 
too MgCl 
25 CaCl, Pepys D 0.98 
too MgCl 
50 See ieee eos E 0. 21 
too MgC] 
00 CaCl, a a aN Sal iy F 0.07 
50 MgCl 
ren CaCl, ages G 0.00 
25 MgCl 
t60 CaCl, Sennen Seat H 0.00 
to MgCl 
I0o CaCl, et wal ae ui 0,00 
5 MgCl 
100 CaCl, ee aie ale ees J 0.00 
TOD (aCe. K 0.49 
By an examination of the curve drawn on the basis of table I (fig. 1), 
Wwe are confronted by the very striking instance of no antagonism 
between the two salts employed. On the contrary, there is a constant 
increase of the toxic properties of each when the other is added to it 
In increasing amounts. In this exceptional behavior, so far as I 
Can ascertain, B. subtilis (and probably all the ammonifiers) stand 
alone when their physiological efficiency in such salt mixtures is 
compared with that of the higher plants and animals. No instance 
of such behavior on the part of any member of the latter two groups 
of organisms has come to my notice in reviewing the results of similar 
researches on animals and the higher plants. 
We find among plants the well-known researches of LOEW’ 
and his pupils, and later the researches of KEARNEY and CAMERON, 
which show the strong antagonism between calcium and magnesium. 
7 Bull. 45, Bureau Pl. Ind., U.S. Depart. Agric.; also Lozw and Aso, Bull. Coll. 
Agric. Tokyo 7:no. 3+ 1907. 
* Report 71, U. S. Depart. Agric. 
