46 BOTANICAL GAZETTE [JANUARY 
CaCl, was injected intravenously in the ear vein. When only 2° 
had thus been injected, the rabbit was again breathing normally, 
and when 8° had been given, the animal sat up and appeared entirely 
recovered, except for a stiffness in the hind legs. 
In these experiments, some of which were even more striking than 
the one cited, MettzeR and AUER employed, besides the chlorids 
of calcium and magnesium, the acetate and nitrate of the former, and 
the acetate, nitrate, and sulfate of the latter, and the same strong 
antagonism was noted in all cases. 
In addition to the confirmation of the results obtained above in my 
_ experiments with the same material, one series was also carried out 
with a culture of B. subtilis obtained fror an entirely different source, 
and the salt solutions made up from a different grade of chemically 
pure salt. As can be seen from the following table, the results fully 
TABLE II 
Numbers in first column refer to c.c. of 0. 35m solutions 
Citsare whois | Taste care | calinrek ag 
OO PRC eas A 4.76 
too MgCl, 
CaCl, i ee B 4.48 
too MgCl 
on ct Ba ages S 4.20 
too MgCl 
a5 CaCl, eee D 3-78 
too MgCl, 
50 CaCl, net. E 3.64 
too MgCl 
Ioo CaCl, ; ie at ee pa F 3- a2 
50 MgCl 
100 CaCl, } pie ata ck whale a G 3: 08 
25 MgCl 
a5 CaCl, Wea ees H 3-29 
10 MgCl, 
100 CaCl, fo [ 3-43 
5 MgCl, } 
100 CaCl, 5 Sie ae eae J 3° 57 
100) CAC a K 3.78 
confirm those above given, and though the absolute amounts are 
different, the results are relatively the same (see also jig. 1). 
