48 BOTANICAL GAZETTE [JANUARY 
Series II. CaCl, versus NaCl 
In this series the solutions were made up in the same way asin the 
preceding one, and the same concentration of salts and peptone was 
employed, the only difference being that CaCl, and NaCl were 
tested instead of CaCl, and MgCl,. The incubation was carried out 
for two and one-half days at 28—29° C., and the distillations and 
determinations were made as above described. The table of results 
follows: 
TABLE Iil 
Numbers in first column refer to c.c. of 0.35 solutions 
Culture solutions ee 
Too NACI Cae, A 14.48 
too NaCl B 
Ee og Ware 10.94 
too NaCl 
= cut Ps C 8. 46 
too NaCl 
25 CaCl, ; Pees ee D 8.18 
too NaCl 
50 CaCl, ‘ 5 oe nee ee E 6.64 
Ioo NaCl F 
too CaCl, § °° 0700" 5.99 
50 NaCl 
ies ot oe a ee G 2.54 
25 NaCl 
Soa H ¥§3 
to NaCl 
too CaCl, § I 0.07 
5 NaCl 
100 CaCl, ; ge SE J 0.00 
00 CaClg K 0.00 
In the curve drawn on the basis of the foregoing table (fig. 2) igi 
note again the striking instance of the lack of antagonism for B. subtilis 
of two salts which showed a strong antagonism in all experiments 0D 
animals and plants thus far carried out. OsTERHOUT’® showed, 
for example, that a strong antagonism exists between NaCl and | 
CaCl, for wheat, and that the curve obtained there was very similar < 
© Bor. GAZETTE 48:98-104. 1909. 
