176 BOTANICAL GAZETTE [MARCH 
found but two instances. All prothallia examined at a later stage 
were of the third kind. 
The archegonial cushion is somewhat thicker than that usually 
figured for polypods, and the archegonia project from it in all direc- 
tions except from the dorsal surface. The development seems to 
correspond to that reported for polypods (jigs. 26-28). There are 
two neck canal nuclei (jig. 28); occasionally four are found, but with 
no walls (jig. 29). 
Aneimia 
SPORE COATS 
The sporangia studied for the development of the spore coat were 
those of Aneimia hirsuta (L.) Sw., in which, the spores show parallel 
ridges set with spines. The material, which had been killed in, 70 
per cent. alcohol and formalin, was imbedded in paraffin and the 
sections stainéd, like those of Lygodium, with safranin and gentian 
violet. 
The earlier stages in the formation of the spore coats agree with 
those in Lygodium except that the mother cell wall is not so delicate, 
and traces of it may still be seen after the walls of the spores appear 
in the tetrad. When the exine begins to show differentiation into 
red and yellow parts, ridges appear on the outer surface and the red 
is largely confined to these ridges, only a line of it appearing near the 
inner edge of the coat (jig. 30). Stages were found before the one 
represented and with barely perceptible ridges, and later ones in 
which they were more prominent. No trace of granules in the cyto- 
plasm can be seen, though the nuclei have an appearance of degenera- 
tion, comparable to that in the same stage of Lygodium. A surface 
view of the coat at this time shows ridges but none of the spines of the 
mature coat. 
That these spines are built up on the ridges by the activity of the 
tapetal protoplasm seems evident from stages like that shown in fig. 37- 
The exine shows the red and yellow, but the spines stain purple, and 
they are very easily pulled away from the ridges. Later these spines 
change their nature, beginning at the part nearest the ridge, and show 
the yellow stain except just at the tip. 
In the case of Aneimia hirsuta, then, the epispore consists of these 
