ae LIPMAN—SOLUTIONS FOR BACTERIA — 209 
water peptone solution when compared with the distilled water 
culture is possibly due to the fact that small quantities of toxic salts 
TABLE I 
ALL QUANTITIES GIVEN REFER TO CUBIC CENTIMETERS OF 0.35 SOLUTIONS 
PEPTONE CONTENT 0.91 PER CENT. 
Number Culture solution S pesto —_ me 
tuition: too NaCl 20.10 
Spee Mle too KCl 15.38 
too NaCl 
eee ae eat { 10 MgCl, f 23.39 i 
too KCl 
We eae 5 CaCl, | 17.90 
aac Ne ua simi in a enoie 42.51 
ee eee en Peptone in tap w; 3% .99 
are dissolved from the metal pipes by the water and thus have an 
unfavorable effect on the growth and development of B. subtilis. 
SERIES II 
The next step taken was to ascertain if salt mixtures containing 
three salts instead of two provide better conditions for ammonification 
TABLE II 
ALL QUANTITIES GIVEN ame TO CUBIC ee OF 0.35 m™ SOLUTIONS 
PEPTONE CONTENT 0.91 PER CENT. 
Number Culture solutions a oA met 
100 NaCl 
Ween to MgCl, 20.63 
o KCl 
too NaCl 
Pee Bees Se ee 1o MgCl, 18.91 
. to CaCl, 
en NaCl 
a o MgCl, 25.88 
a CaCl, 
too NaCl 
CE a 10 i 24.90 
S. i 10 MgCl 22.10 
a too NaCl} . 
1 ew ele its o MgCl, \ 23 39 
