Igto] STOCKBERGER—TOXIC SOLUTIONS AND MITOSIS 419 
The radicles which had been for four hours in 7/188 phenol were 
next examined and all stages of mitosis found. The cytoplasm of 
the cells of the outer layers exhibited a coarsely netted structure due 
to the formation of numerous small vacuoles. Occasional spireme 
nuclei were unduly enlarged, and in a very few cases the nuclei were 
constricted in one diameter, apparently by the development of a 
plasma mass as previously noted. The early stages of the spindle 
were very obscure, but during anaphase the fibers became quite 
distinct. A few cases of late anaphase occurred in which occasional 
chromosomes had not left the nuclear plate, but remained at one 
side of the spindle in line with the row of granules marking the early 
Stages of cell plate formation (fig. 7b). A similar occurrence was 
observed by BiazeK (3) in the cells of root tips of Pisum treated 
with benzol, and in Vicia Faba also by SCHRAMMEN (2'7), who says: 
“Eine nicht seltene Erscheinung bei der plotzlichen Einwirkung 
von hohen Temperaturen ist das Nichterfassen von Chromosomen 
durch die Spindelfasern und das Zuriickbleiben einzelner Chromo- 
Somen bei dem raschen Transport zu den Spindelpolen.” In a few 
cases the chromosomes at one pole of a spindle had diverged and 
formed two groups, to each of which a portion of the spindle 
extended (jig. 7c). SABLINE (26), who produced similar phenomena 
y the use of sulfate of quinine, says, “Sur quelques figures multi- 
polaires on peut voir deux et quelquefois trois fuseaux.” In the 
material treated with phenol, however, multipolar spindles occur so 
rarely that there seems to be no good ground for assuming that they 
are produced by the action of this reagent. 
After an interval of 21 hours in m/188 phenol there were no mitoses. 
The nuclei were all in the resting stage and for the most part contained 
but a single nucleolus. General disorganization of the cells of the 
root tips had begun. 
Tips of radicles were treated for 20 minutes with 10/94 normal 
phenol and then transferred to distilled water for four hours. At 
the end of this time all nuclear activity had ceased. In most cases 
the nucleus was enlarged, and in the field of the microscope appeared 
as a light area against the darker background of the cytoplasm of 
the cell (fig. 7d). The chromatin had almost entirely disappeared 
from the nuclear network. Very prominent in the nucleus appeared 
