422 BOTANICAL GAZETTE [JUNE 
The cytoplasm of many cells was plasmolyzed, while that of others 
exhibited a coarse web or net structure, but the cells themselves 
retained their shape and the outer layers were not disintegrated. 
In the tips of radicles acted on by a o.1 per cent solution for three 
hours all stages of division were observed. The achromatic figures 
were very distinct, and nuclei with two nucleoli occurred frequently. 
After six hours in this concentration the cells of the outer layers were 
dead and the cell walls had collapsed. There were some spireme 
nuclei in the inner periblem and a few nuclei were at anaphase. The 
large deeply staining nucleoli were prominent features of all nuclei 
not disorganized. 
The cells of the outer layers of root tips in o.or per cent strychnin 
for three hours were mostly dead and collapsed. Division figures 
occurred occasionally in the plerome and were frequent in the inner 
periblem cells. The spindle fibers of anaphase were clear and dis- 
tinct, but no polar caps were observed in spireme nuclei. The cell 
plate was regularly laid down in late anaphase and two nucleoli were 
frequent in resting nuclei. After five hours in the o.o1 per cent 
solution some division figures were present. The number of spireme 
nuclei in proportion to those in the later division stages was greater 
than at the end of three hours. Very few nuclei were in late anaphase. 
After 20 hours the area of dead cells included all but those of the 
inner periblem at some distance from the apex of the tip. No defor- 
mation of the nuclei occurred. Even in cells in which the cytoplasm 
was disorganized, the nuclei frequently retained their normal shape 
and general appearance. Growth and nuclear activity seemed to 
have been arrested by the gradual failure of cytoplasmic activity. 
The radicles exposed for ten minutes to the strychnin solutions, 
then transferred to distilled water, were next considered. After ten 
minutes in 1 per cent solution and three hours in water the cells of 
the outer layer were dead. In many of the plerome and inner 
periblem cells containing division figures the cytoplasm was vacuolate, 
but the figures were not disturbed. All stages of division were 
observed, but there were no aberrant mitoses. 
After six hours in this concentration further disintegration of the 
outer cells had taken place. Normal division figures persisted in 
the plerome and inner periblem cells, and a few rare spireme nuclei 
