1920] 



W A LKER—C YA Til US 



3 



a fresh crop of fruit bodies usually developed after each watering. 

 No basidiocarps were found upon cultures kept in the dark, no 

 matter how long the cultures were allowed to remain there. The 

 best results were obtained by keeping the cultures in the dark for 

 three or four months (or longer) , until the culture medium seemed 

 thoroughly permeated with mycelium, and then adding water 

 just before placing them in the light, where fruit bodies developed 

 in a few weeks. The most abundant development of basidiocarps 



}■»*■-+**. :J 



Fig. i. — A, mature spores; 



germination spores; C, branching and nuclear 



conditions in older hypha; D-H, development of clamp connections (x l -x 4 ) and 

 relation of clamps to branching; /, anastomosing hyphae. 



occurred in cultures exposed in an east window, where direct sun- 

 light reached them during a few hours daily. 



Hangixg-drop cultures. — Hanging-drop cultures of spores 

 were made repeatedly, but usually spore germination did not take 

 place for some reason. In a few cases, however, especially in one 

 case in which spores from basidiocarps developed in pure cultures 

 were used, abundant germinations in water were obtained. The 

 germ tube usually came from the end of the spore and remained 

 unbranched for some time. In a few cases branching very near 



