262 



BOTANICAL GAZETTE 



[OCTOBER 



At this time A showed the greatest development, both as to stage 

 and number of buds (eight) developing; B showed two buds 



Fior 



6 



developing; C and D none; E six; F one; and C none, 

 shows the plant fifteen days after beginning of the experiment. 

 At this time in A seventeen buds out of nineteen had developed, 

 and sixteen had produced shoots as well as roots; in B fourteen 

 buds out of sixteen submerged had developed, thirteen with both 

 shoots and roots; in C four buds out of nineteen submerged had 

 developed, all with both shoots and roots; in D two buds out of ten 



Fig. 



Fig. 6 



submerged had developed, one with shoot and roots; in E ten buds 

 out of sixteen submerged had developed, all with shoots and roots; 

 in F eight buds out of sixteen submerged had developed, three shoots 

 and roots, five roots only; in seven buds out of twelve submerged 

 showed some slight development, but only two showed shoots, the 

 others roots only. 



In this and other experiments described, some of the isolations 

 on leaves distant from the experimental leaf may not be the result 

 of the cooling, but merely such as occur on most, plants at this 

 season, when leaves are submerged. In all the series, however, 

 it is evident that the greatest degree of isolation, both as regards 



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